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. 2024 Dec 19;19(12):e0315892. doi: 10.1371/journal.pone.0315892

Fig 2. Sodium butyrate does not impact the levels of proteins and acetylation of proteins after 20 minutes.

Fig 2

(A) Heatmap showing comparison of 2827 detected proteins between control (orange, n = 4) and 15mM SB (green, n = 4) treated fly heads after 20 min. (B) Heatmap comparing 557 detected acetylation sites of 267 proteins between control (orange, n = 4) and 15mM SB (green, n = 4) treated fly heads after 20 min. FC (fold change). Levels of key mitochondrial proteins related to TCA cycle were not significantly altered after 20 min between control (orange, n = 4) and 15mM SB (green, n = 4) group. Genes encoding proteins are as follows: (C) CG3861 (Citrate synthase), (D) CG9244 (Aconitate hydratase), (E) CG7176 (Isocitrate dehydrogenase), (F) CG1544 (2-oxoglutarate dehydrogenase), (G) CG5214 (Dihydrolipoyllysine-residue succinyltransferase), (H) CG7430 (Dihydrolipoyl dehydrogenase), (I) CG11963 (Succinyl-CoA ligase), (J) CG4094 (Fumarate hydratase), (K) CG5362 (Malate dehydrogenase). Error bars indicate ±S.E.M in all graphs. Student’s t-test was performed and detailed statistical information is presented in S3 Table in S1 File. Levels of key mitochondrial proteins were not significantly altered after 20 min between control (orange, n = 4) and 15mM SB (green, n = 4) group. Genes encoding proteins are as follows: (L) Complex I: CG6343 (ND-42), CG2286 (ND-75), (M) Complex II: CG17246 (SdhA), CG6666 (SdhC), (N) Complex III: CG8764 (Ox), CG7361 (Rieske), (O) Complex IV: CG14724 (COX5A), CG9603 (COX7A) and (P) Complex V. Error bars indicate ±S.E.M in all graphs. Student’s t-test was performed and detailed statistical information is presented in S3 Table in S1 File.