Fig. 4.

Predicted alteration to cellular differentiation trajectories in B6J XY Z-del gonads. (A-C) RNA velocity trajectories (arrowheads on black lines) are shown on UMAPs from XY WT, which combines samples from all stages (A), XY Z-del samples from all stages (B) and individual XY Z-del samples at 11.5 and 12.5 dpc (C). The red arrows further indicate the general direction of the individual trajectories. For XY Z-del, in the absence of a Sertoli cluster, the predicted trajectory is towards pre-supporting and SLC cell-types. PS1, pre-supporting 1; PS2, pre-supporting 2; SLC, supporting-like cells.