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. 2024 Oct 14;70(6):362–371. doi: 10.1262/jrd.2024-016

Table 1. Meiotic stages (A) and competence (B) of in vitro-grown bovine oocytes in reconstructed complexes.

(A)

In vitro growth (day) Types of complexes 1) Number of oocytes used Number (%) of oocytes at each stage 5)
FC SC GV ED LD MI AI-TI MII DG
0 3) - 24 11 (46) 13 (54) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0)

14 GCs 18 3 (17) 2 (11) 9 (50) 0 (0) 0 (0) 1 (6) 0 (0) 0 (0) 3 (17)
early MGCs 19 0 (0) 1 (5) 14 (74) 0 (0) 1 (5) 0 (0) 0 (0) 0 (0) 3 (16)
OCGCs 16 1 (6) 0 (0) 11 (69) 2 (13) 0 (0) 0 (0) 0 (0) 0 (0) 2 (13)

In vivo4) - 20 0 (0) 0 (0) 20 (100) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0)

(B)

In vitro growth (day) Types of complexes 2) Number of oocytes used Number (%) of oocytes at each stage 5)
FC SC GV ED LD MI AI-TI MII DG

0 3) - 16 13 (81) 0 (0) 0 (0) 0 (0) 0 (0) 1 (6) 0 (0) 0 (0) 2 (13)

14 GCs 18 1 (6) 0 (0) 0 (0) 0 (0) 0 (0) 6 (33) 0 (0) 11 (61) 0 (0)
early MGCs 20 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 4 (20) 0 (0) 15 (75) 1 (5)
OCGCs 14 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 3 (21) 0 (0) 10 (71) 1 (7)

In vivo4) - 20 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 20 (100) 0 (0)

1) Reconstructed complexes and OCGCs were subjected to in vitro growth culture. 2) Reconstructed complexes and OCGCs were subjected to in vitro maturation culture after in vitro growth culture. DOs were cocultured with GCs and early MGCs. 3) Oocytes were collected from the early antral follicles for culture. 4) Fully grown oocytes were collected from late antral follicles (4–6 mm). 5) FC, filamentous chromatin; SC, stringy chromatin; GV, germinal vesicle I–IV; ED, early diakinesis; LD, late diakinesis; MI, metaphase I; AI-TI, anaphase I and telophase I; MII, metaphase II; DG, degeneration.