Table 1. Meiotic stages (A) and competence (B) of in vitro-grown bovine oocytes in reconstructed complexes.
(A) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
In vitro growth (day) | Types of complexes 1) | Number of oocytes used | Number (%) of oocytes at each stage 5) |
||||||||
FC | SC | GV | ED | LD | MI | AI-TI | MII | DG | |||
0 3) | - | 24 | 11 (46) | 13 (54) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
14 | GCs | 18 | 3 (17) | 2 (11) | 9 (50) | 0 (0) | 0 (0) | 1 (6) | 0 (0) | 0 (0) | 3 (17) |
early MGCs | 19 | 0 (0) | 1 (5) | 14 (74) | 0 (0) | 1 (5) | 0 (0) | 0 (0) | 0 (0) | 3 (16) | |
OCGCs | 16 | 1 (6) | 0 (0) | 11 (69) | 2 (13) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 2 (13) | |
In vivo4) | - | 20 | 0 (0) | 0 (0) | 20 (100) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
(B) | |||||||||||
In vitro growth (day) | Types of complexes 2) | Number of oocytes used | Number (%) of oocytes at each stage 5) |
||||||||
FC | SC | GV | ED | LD | MI | AI-TI | MII | DG | |||
0 3) | - | 16 | 13 (81) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (6) | 0 (0) | 0 (0) | 2 (13) |
14 | GCs | 18 | 1 (6) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 6 (33) | 0 (0) | 11 (61) | 0 (0) |
early MGCs | 20 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 4 (20) | 0 (0) | 15 (75) | 1 (5) | |
OCGCs | 14 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 3 (21) | 0 (0) | 10 (71) | 1 (7) | |
In vivo4) | - | 20 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 20 (100) | 0 (0) |
1) Reconstructed complexes and OCGCs were subjected to in vitro growth culture. 2) Reconstructed complexes and OCGCs were subjected to in vitro maturation culture after in vitro growth culture. DOs were cocultured with GCs and early MGCs. 3) Oocytes were collected from the early antral follicles for culture. 4) Fully grown oocytes were collected from late antral follicles (4–6 mm). 5) FC, filamentous chromatin; SC, stringy chromatin; GV, germinal vesicle I–IV; ED, early diakinesis; LD, late diakinesis; MI, metaphase I; AI-TI, anaphase I and telophase I; MII, metaphase II; DG, degeneration.