Table 1. Development and mutation of zygotes edited with guide RNAs (gRNAs) targeting the growth hormone receptor (GHR) and glycoprotein alpha-galactosyltransferase 1 (GGTA1).

Target	No. of zygotes	No. (%) of zygotes developed to blastocysts	No. of blastocysts examined	No. (%) of gene-edited blastocysts *
				WT	Mosaic	Average efficiency
GHR	200	25 (12.5 ± 2.5)	17	15 (88.2)	2 (11.8)	9.1
GGTA1	196	31 (15.8 ± 3.3)	16	12 (75.0)	4 (25.0)	16.4

Zona pellucida-free zygotes collected 10 h after in vitro fertilization were co-incubated with guide RNAs (gRNAs) and Lipofectamine 2000 (LP2000) for 5 h and cultured in 4-well culture plates. Four replicates were performed. * Percentages were calculated by dividing the number of mutant blastocysts by the number of blastocysts examined. Average efficiency indicates the proportion of indel mutation events in mutant blastocysts, as determined using the Tracking of Indels by Decomposition (TIDE) analysis.