Table 2. In vitro development of IVF embryos for 96 h PI from various ages of C57BL/6J mice.
| Age (weeks) | No. 2-cell cultured | No. of embryos at 96 h PI (% ± MSE) a
|
Nuclear number of blastocysts |
||
|---|---|---|---|---|---|
| Blastocysts | Expanded blastocysts | No. counted blastocysts | Nuclear number | ||
| 3 | 94 | 65 (69 b ± 3) | 60 (64 b ± 4) | 27 | 58.2 b ± 3.1 |
| 4 | 96 | 89 (93 c ± 1) | 88 (92 c ± 1) | 15 | 62.1 bc ± 3.7 |
| 5 | 36 | 35 (97 c ± 3) | 34 (95 c ± 5) | 11 | 63.5 bc ± 5.0 |
| 6 | 36 | 35 (97 c ± 3) | 35 (97 c ± 3) | 13 | 64.2 bc ± 4.0 |
| 8 | 107 | 103 (96 c ± 1) | 103 (96 c ± 1) | 40 | 66.7 c ± 1.7 |
| 10 | 125 | 121 (97 c ± 1) | 121 (97 c ± 1) | 60 | 65.1 c ± 1.5 |
a Percentage and mean standard error (MSE) of 2-cell embryos cultured. bc Percentages or numbers with different superscripts in the same column were significantly different (P < 0.05).