Fig. 6.

Identification of putative candidate genes for flower development and sex determination in hemp. The Venn diagrams illustrate the number of genes overlapping between the male- or female-specific modules identified through WGCNA, sex chromosome linkage, and DEGs identified in L4M versus L9F (A) and L2M versus L2F (B). Numbers in white text indicate putative flower development genes for male or female hemp plants that are not located on sex chromosomes. Numbers in black underlined text indicate putative sex-determination genes for male (overlap of putative ChrY, male-specific module from WGCNA, and male-biased genes from DEGs) or female (overlap of putative ChrX, female-specific module from WGCNA, and female-biased genes from DEGs) hemp plants. In the L4M versus L9F comparison (A), the combined counts of 17 and 16 represent all female flower development genes, whereas the combined counts of 35 and 107 represent all male flower development genes (see Supplementary Fig. S8).