Table 1.

The primers, reporter molecule and gRNAs used in this study.

Primers/gRNAs a	Sequences and modifications b	Length (nt) c	Genes
ORF1ab-F1	5’-TGCATCGTGTTGTCTGT-3’	17	ORF1ab
ORF1ab-F2	5’-TTACACCGCAAACCCGT-3’	17
ORF1ab-CP1	5′- AGCACAAGTTGTAGGTATTTGTACATTCCCGTTGCCACATAGATCA −3’	46
ORF1ab-CP2	5′- CGTCTGCGGTATGTGGAAAGATTGTGCATCAGCTGACTG −3’	40
ORF1ab-C1	5’-AGCACAAGTTGTAGGTATTTGTACA-3’	25
ORF1ab-C2	5’-CGTCTGCGGTATGTGGAAAG-3’	20
ORF1ab-D1	5’-CACAAAATCCTTTAGGATTTG-3’	21
ORF1ab-D2	5’-CTGTAGTTGTGATCAACTC-3’	19
ORF1ab-R1	5’-AAAACCCACAGGGTCAT-3’	17
ORF1ab-R2	5’-CTTAAAAACACAGTCTGTAC-3’	20
ORF1ab-gRNA	5’-GUCUAGAGGACAGAAUUUUUCAACGGGUGUGCCAAUGGCCACUUUCCAGGUGGCAAAGCCCGUUGAGCUUCUCAAAUCUGAGAAGUGGCACCCGUUGCCACAUAGAUCAUC-3’	111
N-F1	5’-ATTGAACCAGCTTGAGAGCA-3’	20	N
N-F2	5’-GCAATTTGCGGCCAATGT-3’	18
N-CP1	5’-TTGCCGAGGCTTCTTAGAAGCCAGGCCAACAACAACAAGGC-3’	41
N-CP2	5’-AACACAAGCTTTCGGCAGACGTTGATTAGTTCCTGGTCCCCA-3’	42
N-C1	5’-TTGCCGAGGCTTCTTAGAAGCC-3’	22
N-C2	5’-AACACAAGCTTTCGGCAGACGT-3’	22
N-D1	5’-CAGCAGCAGATTTCTTAGTGACAG-3’	24
N-D2	5’-GGTCCAGAACAAACCCAAGG-3’	20
N-R1	5’-TTTCATTGTATGCTTTAGTGGCA-3’	23
N-gRNA	5’-GUCUAGAGGACAGAAUUUUUCAACGGGUGUGCCAAUGGCCACUUUCCAGGUGGCAAAGCCCGUUGAGCUUCUCAAAUCUGAGAAGUGGCACCAUUGUAUGCUUUAGUGGCA-3’	111
Fluorescent reporter	5’-FAM-TTATTAT-BHQ1-3’	7
Lateral flow reporter	5’-FAM-TTATTAT-Biotin-3’	7

^aORF1ab, open reading frame 1a/b; N, nucleoprotein gene.

^bORF1ab-CP1 and N-R1primers were modified in linker region with PAM site (TTC and TTT marked in red).

^cnt, nucleitide.