FIGURE 1.

A time-calibrated phylogeny of 3,167 NFF species (or 51% of NFF) allocated to 741 genera (or 86% of NFF), illustrating evolutionary relationships among genera across major lineages. This tree was estimated using RAxML-HPC and time-calibrated through congruification (Eastman et al., 2013) employing divergence tree estimates from Hughes et al. (2018). The tree resulted from alignments of 51 orthologous markers, totaling 43,850 bp, with 21,223 sequences and 5,984 terminals. Vertical bars indicate the complete species per genus represented on the tree. All GenBank accession numbers, alignments, species-level relationships, divergence clade estimates and species representativeness percentages are provided in the Supplementary Material. This phylogeny, as well as one with nodes bearing bootstrap values below 70% collapsed, is available as a Newick string in the Supplementary Material and can be found in the “phy” folder.