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. 2024 Dec 19;9:40. doi: 10.1038/s41536-024-00384-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — a Schematic representation of the transgenic plasmid pMlc2-H2B-mCherry which harboring the expression cassette of H2B-mCherry (H2C) fusion protein under control of Xenopus mlc2 promoter. b Whole-mount bright-field (upper), epifluorescence (middle), and merged (lower) images showing mCherry expression in Wild-type (WT, left) and F1 Tg(mlc2:H2C) (right) tadpoles. Arrows indicate heart location. c Representative image of the living heart in F1 Tg(mlc2:H2C) tadpole. Red color means mCherry expression specifically in cardiomyocyte nuclei. d Whole-mount bright-field (upper) and epifluorescence (lower) images showing mCherry expression in the adult hearts from WT (left) and F1 Tg(mlc2:H2C) (right) frogs. e Immunostained images for mCherry (red) expression in the adult heart from F1 Tg(mlc2:H2C) frog. DAPI was used as a nuclear stain (blue). Left panel, whole cardiac section in low-magnification. Right panels, high-magnification images of the atrial (upper) and ventricular (lower) regions white boxed in the whole cardiac section. This figure was created by Photoshop Image 12 software using our own data in the work.