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. 2024 Dec 19;9:40. doi: 10.1038/s41536-024-00384-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — a Immunostaining for mCherry (red) and Ki67 (green) expression in the apex of adult hearts from F1 Tg(mlc2:H2C) frogs at the indicated time points after resection. DAPI was used as a nuclear stain (blue). b Quantification of mCherry⁺Ki67⁺ cells in ventricular apex during heart regeneration within 30 days. Data are presented as mean ± SEM (n = 3 hearts for sham, 5 ~ 8 hearts for injured groups). *p < 0.05, ****p < 0.0001 versus sham. ^###p < 0.001, ^####p < 0.0001 (one-way ANOVA test). c Representative images of mCherry⁺Ki67⁺ cells in the whole apical regions at 7 and 30 dpr. Magnified Z-stack confocal images of mCherry⁺Ki67⁺ cells are shown in right panel. d Immunostaining for mCherry (red) and pH3 (green, antibody from CST, #9701) expression in the apex of adult hearts from F1 Tg(mlc2:H2C) frogs at the indicated time points after resection. DAPI was used as a nuclear stain (blue). e Quantification of mCherry⁺pH3⁺ cells in ventricular apex during heart regeneration within 30 days. Data are presented as mean ± SEM (n = 3 hearts for sham, 5 hearts for injured groups). **p < 0.01, ****p < 0.0001 versus sham. ^#p < 0.05, ^####p < 0.0001 (one-way ANOVA test). f Representative images of mCherry⁺pH3⁺ cells in the whole apical regions at 7 and 30 dpr. Magnified confocal images of mCherry⁺pH3⁺ cells are shown in right panel.