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. 2024 Dec 19;7:302. doi: 10.1038/s42004-024-01392-z

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© The Author(s) 2024, corrected publication 2024

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Fig. 5 — Reaction of tetrazine 2b and TCO 10, resulting in instantaneous formation of cyclized elimination product 14, formed either through pathway A or B. Control experiment 1: Reaction of 2b with 9 in presence of sodium nitrite to in situ oxidize 4,5-tautomer to pyridazine, preventing 1,4-elimination; lack of release supports A. Control experiment 2: Reaction of 2b with TCO ether 23, leading to rapid and quantitative release of hydroxyl, supporting A.