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. 2024 Dec 19;7:1679. doi: 10.1038/s42003-024-07377-x

Fig. 3. Chemerin-9 alleviates ferroptosis in the kidney to attenuate renal I/R injury.

Fig. 3

A Western blot analysis of GPX4, ACSL4, SLC7A11, IL-6, and TNFα abundance in the kidneys. β-Actin was used as a loading control. n = 3 (independent experiments). B Quantified data from the western blots shown in (A). C, D Serum TNFα (C) and IL-6 (D) levels were reduced by C9 in I/R model mice. n = 5 mice/group. E Representative Prussian blue staining images showing iron deposition in the kidneys. Arrows indicate positive signals for iron staining. Scale bars = 100 μm. F Representative transmission electron microscopy (TEM) images showing mitochondrial structures in the kidneys. Arrows indicate damaged mitochondria. Scale bars = 5 μm. Lipid hydroperoxide (LPO; G) and malondialdehyde (MDA; H) levels were decreased by C9. n = 3 mice/group. Data are presented as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, by one-way ANOVA with Tukey’s multiple comparison test.