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. 2024 Dec 19;7:304. doi: 10.1038/s42004-024-01388-9

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© The Author(s) 2024

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Fig. 5 — A and D DNA recovery from qPCR after each round of selection against PADI4 and PADI4 C645A, respectively, from biotinylated beads (negative) and PADI4 beads (positive), compared to the input DNA from each round. B and E Enrichment in warhead-containing peptides for PADI4 and PADI4 C645A selections, respectively. Percentage of sequences from each round of selection which do not contain an internal methionine residue, which encodes the FAO warhead. C Enrichment of key sequences at the 5^th round of selection from selection one (hashed bars) and selection two (filled bars), where cP4_13 from selection two has high sequence homology with cP4_2 from selection one. F Low levels of enrichment of key sequences over the six rounds of selection against PADI4 C645A.