Table 1.
Sequences of peptides synthesised after the first selection, and the rationally designed PADI4_3_H4(2)
| Peptide Name | Sequence | IC50 Values (µΜ) | |
|---|---|---|---|
| 0 h preincubation | 1 h preincubation | ||
| cP4_2 | yIWGL(2)D(2)SCG | >50 | 0.87 ± 0.06 |
| cP4_4 | ySKYD(2)RSPRDCG | 4.4 ± 0.07 | 0.070 ± 0.004 |
| cP4_7 | yVYS(2)KEWKYCG | 8.0 ± 1.2 | 0.16 ± 0.02 |
| cP4_10 | yWY(2)NWDFNKRCG | 3.1 ± 0.01 | 0.093 ± 0.002 |
| cP4_15 | yLD(2)HYSSKLYCG | 1.6 ± 0.03 | 0.052 ± 0.002 |
| cP4_165 | yVY(2)DCEWINRAG | 11.8 ± 4.9 | 0.17 ± 0.01 |
| PADI4_3_H4(2) | yRD(2)HYRHPKYCG | 2.0 ± 0.01 | 0.065 ± 0.007 |
Where (2) is the FAO warhead and y is chloroacetyl-D-tyrosine which is cyclised with the cysteine residue in each peptide. Their corresponding IC50 values from COLDER assay with or without 1 h preincubation of peptide and PADI4 are also shown. Data shows mean ± SEM of two independent replicates.