Table 2.
Kinetic parameters of peptides. Both COLDER and SPR values show mean ± SEM from three independent replicates
| SPR | Colders | |||||
|---|---|---|---|---|---|---|
| Ki (µM) | kinact (min-1) | kinact/Ki (M-1 min-1) | KI (µM) | kinact (min-1) | kinact/KI (M-1 min-1) | |
| cP4_2 | 1.3 ± 0.5 | 0.12 ± 0.1 | 92,000 | - | - | - |
| cP4_4 | 0.12 ± 0.01 | 0.11 ± 0.1 | 909,000 | 1.9 ± 0.1 | 0.13 ± 0.01 | 74,000 |
| cP4_7 | 1.6 ± 0.1 | 0.55 ± 0.02 | 343,000 | 4.3 ± 1.1 | 0.077 ± 0.052 | 18,000 |
| cP4_10 | 0.12 ± 0.1 | 0.23 ± 0.01 | 2,007,000 | 1.5 ± 0.3 | 0.13 ± 0.03 | 87,000 |
| cP4_15 | 0.16 ± 0.03 | 0.25 ± 0.04 | 1,594,000 | 0.78 ± 0.06 | 0.17 ± 0.02 | 213,000 |
| cP4_165 | 0.62 ± 0.06 | 0.066 ± 0.007 | 107,000 | 8.9 ± 1.1 | 0.16 ± 0.01 | 17,000 |
| PADI4_3 H4(2) | 0.015 ± 0.002 | 0.061 ± 0.011 | 4,083,000 | 1.1 ± 0.2 | 0.16 ± 0.03 | 149,000 |