Figure 4.

Apelin/APLNR exacerbated hepatic lipid accumulation and fibrosis in MASLD mice. (A) HE staining of liver paraffin sections shows pathological changes in the liver after intraperitoneal injection of apelin or ML221. (B) Oil Red O staining of mouse liver frozen sections. (C) Masson's trichrome staining of mouse liver paraffin sections. (D-F) Blood was collected from each group of mice after 9 weeks of feeding, and serum was separated for the measurement of TC, TG, and glucose using assay kits (n=4). The P values are calculated by one-way ANOVA followed by Tukey's multiple comparisons tests (D-F). *P < 0.05; **P < 0.01; ***P < 0.001