Figure 6.

Apelin/APLNR could promote B cell migration and activation. (A-B) Cultured Raji cells were transfected with a plasmid to overexpress APLNR and were treated with apelin recombinant protein in the culture medium. Transcriptome sequencing was conducted along with the control group (n=3). The heatmap and volcano plot of differentially expressed genes are shown. (C) Dot plot of GSEA pathway analysis. (D) GSEA pathway analysis showing extracellular matrix organization pathway and degradation of the extracellular matrix pathway. (E) After overexpressing APLNR in Raji cells through plasmid transfection and adding LPS or apelin to the culture medium, cells were collected for RNA extraction, and RT-PCR was performed to measure the mRNA levels of AHNAK, COL6A3, IL10, CD86, IRF9, and RFX2. The P values are calculated by one-way ANOVA followed by Tukey's multi comparison test (E). *P < 0.05; **P < 0.01; ***P < 0.001