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. 2024 Nov 15;4(6):416–425. doi: 10.1021/acsnanoscienceau.4c00040

Figure 4.

Figure 4

Single-drug NP’s gene restoration and knockdown, and simultaneous gene restoration and knockdown mediated by codelivery dual-drug NPs using mRNA and siRNA in two cell lines. (A,B) Single drug (M)-NPs (0.016 nmol) and empty-NPs (10 μM) treated with HT1080 and MDA-MB-231 cells, and 48 h post-treatment with luciferase (RLU) measured by a plate reader, n = 3 (data represent means ± SD, *p < 0.05). (C) Single-drug (S)-NPs (1 nmol) and empty-NPs (10 μM) treated to GFP+ cells (HT1080 and MDA-MB-231). (D,E) Count of GFP knockdown was analyzed by flow cytometry histogram; no GFP cells were used as a positive control. (F) GFP+ cells (HT1080 and MDA-MB-231) were treated with (M+S)-NPs (∼60 nm size) coloaded with siRNA-GFP (1 nmol) and Luc mRNA (0.016 nmol) for 48 h. (G,H) GFP knockdown count was analyzed via flow cytometry; no GFP cells were used as positive control. (I) Luciferase expression was measured in GFP+ cells by luminescence, n = 3 (data represent means ± SD, *p < 0.05, **p < 0.01).