Figure 8.

Localization of α_i2, sα_i2, and α_i2A327S and the effect of βγ Expression vectors encoding α_i2 (A and B), sα_i2 (C and D), or α_i2A327 (E and F) were transfected alone (A, C, and E) or together with expression vectors for β₁ and γ₂ (B, D, and F) into BHK cells. Proteins were visualized by immunofluorescence microscopy using the EE monoclonal antibody followed by an Alexa 488 conjugate anti-mouse antibody. Bar, 10 μm. G, EE-α_i2-pcDNA3 (lanes 1 and 2) or EE-sα_i2-pcDNA3 (lanes 3 and 4) were transfected into COS-7 cells alone (lanes 1 and 3) or with co-transfection of vectors encoding β₁ and γ₂ (lanes 2 and 4). Immunoblotting with the EE antibody detected α_i2 or sα_i2. Note that sα_i2 expressed alone (lane 3) is very weakly detected and barely visible in this total cell lysate.