Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1973 Nov;136(3):825–828. doi: 10.1042/bj1360825

Preferential synthesis of a membrane-associated protein by free polyribosomes (Short Communication)

Dorrit Lowe 1, T Hallinan 1
PMCID: PMC1166022  PMID: 4780706

Abstract

Isolated free polyribosomes from rat liver appear to synthesize NADPH–cytochome c reductase in vitro four times as efficiently as do membrane-bound polyribosomes. The observed synthesis by the latter could result from contamination with free polyribosomes, but an alternative explanation is also suggested.

Full text

PDF
825

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett J., Scott K. J. Quantitative staining of fraction I protein in polyacrylamide gels using Coomassie brillant blue. Anal Biochem. 1971 Sep;43(1):173–182. doi: 10.1016/0003-2697(71)90122-9. [DOI] [PubMed] [Google Scholar]
  2. Campbell P. N. Functions of polyribosomes attached to membranes of animal cells. FEBS Lett. 1970 Mar 16;7(1):1–7. doi: 10.1016/0014-5793(70)80603-2. [DOI] [PubMed] [Google Scholar]
  3. DALLNER G., ORRENIUS S., BERGSTRAND A. Isolation and properties of rough and smooth vesicles from rat liver. J Cell Biol. 1963 Feb;16:426–430. doi: 10.1083/jcb.16.2.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Fleck A., Begg D. The estimation of ribonucleic acid using ultraviolet absorption measurements. Biochim Biophys Acta. 1965 Nov 8;108(3):333–339. doi: 10.1016/0005-2787(65)90025-0. [DOI] [PubMed] [Google Scholar]
  6. Ganoza M. C., Williams C. A. In vitro synthesis of different categories of specific protein by membrane-bound and free ribosomes. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1370–1376. doi: 10.1073/pnas.63.4.1370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hallinan T., Murty C. N., Grant J. H. The exclusive function of reticulum bound ribosomes in glycoprotein biosynthesis. Life Sci. 1968 Mar 15;7(6):225–232. doi: 10.1016/0024-3205(68)90016-7. [DOI] [PubMed] [Google Scholar]
  8. Hallinan T., Nagley P., Murty C. N., Bennett J., Grant J. H. The use of radioactive choline as a label for microsomal membranes. II. Studies on membrane-ribosome interaction, subfractionation and function. Biochim Biophys Acta. 1969 Apr;173(3):554–563. doi: 10.1016/0005-2736(69)90020-0. [DOI] [PubMed] [Google Scholar]
  9. Hicks S. J., Drysdale J. W., Munro H. N. Preferential synthesis of ferritin and albumin by different populations of liver polysomes. Science. 1969 May 2;164(3879):584–585. doi: 10.1126/science.164.3879.584. [DOI] [PubMed] [Google Scholar]
  10. Ichihara K., Kusunose E., Kusunose M. Effect of triton X-100 and trypsin on NADPH-cytochrome C reductase reconstitutively active in fatty acid omega-hydroxylation. FEBS Lett. 1972 Jan 15;20(1):105–107. doi: 10.1016/0014-5793(72)80028-0. [DOI] [PubMed] [Google Scholar]
  11. Kadenbach B. Biosynthesis of cytochrome c. The sites of synthesis of apoprotein and holoenzyme. Eur J Biochem. 1970 Feb;12(2):392–398. doi: 10.1111/j.1432-1033.1970.tb00864.x. [DOI] [PubMed] [Google Scholar]
  12. Kuriyama Y., Omura T., Siekevitz P., Palade G. E. Effects of phenobarbital on the synthesis and degradation of the protein components of rat liver microsomal membranes. J Biol Chem. 1969 Apr 25;244(8):2017–2026. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lowe D., Reid E., Hallinan T. Importance of centrifugation time and homogenate concentration for isolating rat liver free polysomes: Effect of fasting. FEBS Lett. 1970 Jan 26;6(2):114–116. doi: 10.1016/0014-5793(70)80015-1. [DOI] [PubMed] [Google Scholar]
  15. PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
  16. Ragnotti G. Effect of a modified separation procedure on the size and protein-synthetic activity of membrane-bound liver polyribosomes. Biochem J. 1971 Dec;125(4):1049–1058. doi: 10.1042/bj1251049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ragnotti G., Lawford G. R., Campbell P. N. Biosynthesis of microsomal nicotinamide-adenine dinucleotide phosphate-cytochrome c reductase by membrane-bound and free polysomes from rat liver. Biochem J. 1969 Apr;112(2):139–147. doi: 10.1042/bj1120139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Redman C. M. Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver. J Biol Chem. 1969 Aug 25;244(16):4308–4315. [PubMed] [Google Scholar]
  19. Shuster L., Schrier B. K. Acrylamide gel-sucrose gradient electrophoresis: a useful preparative method. Anal Biochem. 1967 May;19(2):280–293. doi: 10.1016/0003-2697(67)90164-9. [DOI] [PubMed] [Google Scholar]
  20. Takagi M., Tanaka T., Ogata K. Functional differences in protein synthesis between free and bound polysomes of rat liver. Biochim Biophys Acta. 1970 Sep 17;217(1):148–158. doi: 10.1016/0005-2787(70)90131-0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES