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. 1973 Nov;136(3):825–828. doi: 10.1042/bj1360825

Preferential synthesis of a membrane-associated protein by free polyribosomes (Short Communication)

Dorrit Lowe 1, T Hallinan 1
PMCID: PMC1166022  PMID: 4780706

Abstract

Isolated free polyribosomes from rat liver appear to synthesize NADPH–cytochome c reductase in vitro four times as efficiently as do membrane-bound polyribosomes. The observed synthesis by the latter could result from contamination with free polyribosomes, but an alternative explanation is also suggested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett J., Scott K. J. Quantitative staining of fraction I protein in polyacrylamide gels using Coomassie brillant blue. Anal Biochem. 1971 Sep;43(1):173–182. doi: 10.1016/0003-2697(71)90122-9. [DOI] [PubMed] [Google Scholar]
  2. Campbell P. N. Functions of polyribosomes attached to membranes of animal cells. FEBS Lett. 1970 Mar 16;7(1):1–7. doi: 10.1016/0014-5793(70)80603-2. [DOI] [PubMed] [Google Scholar]
  3. DALLNER G., ORRENIUS S., BERGSTRAND A. Isolation and properties of rough and smooth vesicles from rat liver. J Cell Biol. 1963 Feb;16:426–430. doi: 10.1083/jcb.16.2.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Fleck A., Begg D. The estimation of ribonucleic acid using ultraviolet absorption measurements. Biochim Biophys Acta. 1965 Nov 8;108(3):333–339. doi: 10.1016/0005-2787(65)90025-0. [DOI] [PubMed] [Google Scholar]
  6. Ganoza M. C., Williams C. A. In vitro synthesis of different categories of specific protein by membrane-bound and free ribosomes. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1370–1376. doi: 10.1073/pnas.63.4.1370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hallinan T., Murty C. N., Grant J. H. The exclusive function of reticulum bound ribosomes in glycoprotein biosynthesis. Life Sci. 1968 Mar 15;7(6):225–232. doi: 10.1016/0024-3205(68)90016-7. [DOI] [PubMed] [Google Scholar]
  8. Hallinan T., Nagley P., Murty C. N., Bennett J., Grant J. H. The use of radioactive choline as a label for microsomal membranes. II. Studies on membrane-ribosome interaction, subfractionation and function. Biochim Biophys Acta. 1969 Apr;173(3):554–563. doi: 10.1016/0005-2736(69)90020-0. [DOI] [PubMed] [Google Scholar]
  9. Hicks S. J., Drysdale J. W., Munro H. N. Preferential synthesis of ferritin and albumin by different populations of liver polysomes. Science. 1969 May 2;164(3879):584–585. doi: 10.1126/science.164.3879.584. [DOI] [PubMed] [Google Scholar]
  10. Ichihara K., Kusunose E., Kusunose M. Effect of triton X-100 and trypsin on NADPH-cytochrome C reductase reconstitutively active in fatty acid omega-hydroxylation. FEBS Lett. 1972 Jan 15;20(1):105–107. doi: 10.1016/0014-5793(72)80028-0. [DOI] [PubMed] [Google Scholar]
  11. Kadenbach B. Biosynthesis of cytochrome c. The sites of synthesis of apoprotein and holoenzyme. Eur J Biochem. 1970 Feb;12(2):392–398. doi: 10.1111/j.1432-1033.1970.tb00864.x. [DOI] [PubMed] [Google Scholar]
  12. Kuriyama Y., Omura T., Siekevitz P., Palade G. E. Effects of phenobarbital on the synthesis and degradation of the protein components of rat liver microsomal membranes. J Biol Chem. 1969 Apr 25;244(8):2017–2026. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lowe D., Reid E., Hallinan T. Importance of centrifugation time and homogenate concentration for isolating rat liver free polysomes: Effect of fasting. FEBS Lett. 1970 Jan 26;6(2):114–116. doi: 10.1016/0014-5793(70)80015-1. [DOI] [PubMed] [Google Scholar]
  15. PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
  16. Ragnotti G. Effect of a modified separation procedure on the size and protein-synthetic activity of membrane-bound liver polyribosomes. Biochem J. 1971 Dec;125(4):1049–1058. doi: 10.1042/bj1251049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ragnotti G., Lawford G. R., Campbell P. N. Biosynthesis of microsomal nicotinamide-adenine dinucleotide phosphate-cytochrome c reductase by membrane-bound and free polysomes from rat liver. Biochem J. 1969 Apr;112(2):139–147. doi: 10.1042/bj1120139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Redman C. M. Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver. J Biol Chem. 1969 Aug 25;244(16):4308–4315. [PubMed] [Google Scholar]
  19. Shuster L., Schrier B. K. Acrylamide gel-sucrose gradient electrophoresis: a useful preparative method. Anal Biochem. 1967 May;19(2):280–293. doi: 10.1016/0003-2697(67)90164-9. [DOI] [PubMed] [Google Scholar]
  20. Takagi M., Tanaka T., Ogata K. Functional differences in protein synthesis between free and bound polysomes of rat liver. Biochim Biophys Acta. 1970 Sep 17;217(1):148–158. doi: 10.1016/0005-2787(70)90131-0. [DOI] [PubMed] [Google Scholar]

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