Figure 5.

The effect of prior IO treatment on cytokines and their associations with response. A. Association between prior IO and DCB (Fisher’s exact test, p = 0.4, OR = 0.67). B. Systemic effect of prior IO on circulating cytokines from pre treatment time point. Van elteren tests were performed to evaluate baseline cytokines association with priorIO, adjusted by propensity scores for receiving priorIO. Significance test results are marked with asterisks in heatmap (*:p <0.05, **:p<0.01, ***:p <0.001) (patients with priorIO n = 24; patients without priorIO n= 65). C. AUC of applying three- feature model on IO treated and untreated patients subsets. (patients without priorIO: AUC = 0.8, patients with priorIO: AUC = 0.73). D. Association between response to therapy and CXCL6 levels stratified by prior IO. MWW tests were performed, with asterisks showing statistical significance (*:p <0.05, **:p<0.01, ***:p <0.001). E. Association between response to therapy and IL10 levels stratified by prior IO. MWW tests were performed, with asterisks showing statistical significance (*:p <0.05, **:p<0.01, ***:p <0.001). F. Association between response to therapy and IL23 levels stratified by prior IO. MWW tests were performed, with asterisks showing statistical significance (*:p <0.05, **:p<0.01, ***:p <0.001).