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Obvious damage of testis and spermatozoa in epididymis appeared at 30 days after busulfan treatment. Mice were injected intraperitoneally once with busulfan, and then testes and epididymes were separated for H&E staining (A) and measurement of testis weight (B) and spermatozoa number (D) after 10, 20 and 30 days treatment. Arrow heads indicate abnormal germ cells (A). Body weight was also recorded before and after busulfan treatment (C). NC, negative control; *p < 0.05.
