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Proteomic analysis of busulfan-treated mouse testis. 190 significantly downregulated proteins were identified in busulfan-treated mouse testis using volcano plot analysis (A). Partially downregulated proteins were shown in the clustering heatmap (B). Mice were injected intraperitoneally once with busulfan, and then testes were separated for label-free quantification proteomics after 30 days. NC, negative control.
