Table 3.
Characterisation of Rickettsia spp. isolates from Ambyomma hebraeum and blood from cattle, plus guidelines for minimum similarity (%) by Fournier et al. (2003)
| Sample ID | % identity to closest Rickettsia spp. (i.e., R. africae in all instances) by BLAST search (GenBank accession number) | ||||
|---|---|---|---|---|---|
| gltA | ompA | ompB | Sca4 | 17kDa | |
| Minimum % similarity (≥) | 99.9 | 98.8 | 99.2 | 99.3 | No guidelines for this gene |
| C1 | 99.20 (CP001612.1) | 99.82 (MH751466.1) | 98.69 (KU721071.1) | 99.62 (AF151724.2) | 100 (CP001612.1) |
| C3 | 99.02 (CP001612.1) | 100 (MH751466.1) | 99.52 (KU721071.1) | 98.31 (CP001612.1) | 100 (CP001612.1) |
| C9 | 98.58 (CP001612.1) | 100 (MH751466.1) | 99.52 (KU721071.1) | 98.31 (CP001612.1) | 100 (CP001612.1) |
| BC1 | – | 99.81 (MH751466.1) | – | – | – |
| C12 | – | 99.49 (MH751466.1) | – | – | – |
| C19 | – | 99.66 (MH751466.1) | – | – | – |
| C20 | – | 99.16 (MH751466.1) | – | – | – |
| C21 | – | 98.99 (MH751466.1) | – | – | – |
| R1 | – | – | 99.75 (KU721071.1) | – | – |
| R3 | – | – | 99.75 (KU721071) | – | – |
‘–’ No sequencing results were obtained for this sample