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. 2024 Dec 19;24:411. doi: 10.1186/s12935-024-03604-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — miR-615-3p was upregulated in OS cells and tissue. A. Volcano plot representing the differentially expressed genes in GEO datasets (GSE28423). B. Heatmap showed the differentially expressed genes in GSE28423 downloaded from the GEO database. C. The expression of miR-615-3p in normal and osteosarcoma samples of GSE28423 dataset. D. Representative FISH photographs of the expression patterns of miR-615-3p in human OS tissues. Scale bars = 20 μm. E. Statistical analysis of FISH results based on the expression level of miR-615-3p in T1 stage (n = 9) and T2 stage (n = 31) OS tissues. F. Relative miR-615-3p mRNA levels of MG63, MNNG-HOS, U-2OS and 143B relative to hFOB1.19 cells were determined using qRT-PCR. Results are displayed as mean ± SD, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001