Fig. 7.

miR-615-3p promotes OS growth by targeting SESN2 in vivo. A. Morphologic characteristics of xenograft tumors from different groups (anti-NC, anti-miR-615-3p, anti-miR-615-3p + sh-SESN2) (n = 5). Scale bars = 1 cm. B. The tumor volumes were measured with calipers every 5 days. C. Tumor weights at 20 days were measured in each group (anti-NC, anti-miR-615-3p, anti-miR-615-3p + sh-SESN2). The median, upper, and lower quartiles were plotted, and the whiskers that extend from each box indicate the range of values that were outside of the intra-quartile range (n = 5). D. Tumor volumes at 20 days were measured in each group (anti-NC, anti-miR-615-3p, anti-miR-615-3p + sh-SESN2). The median, upper, and lower quartiles were plotted, and the whiskers that extend from each box indicate the range values that were outside of the intra-quartile range (n = 5). E. Representative images of Ki67 and TUNEL staining in the xenograft tumors from each experimental group of nude mice. A TUNEL positive cell is indicated with an arrow. Scale bars = 50 μm. F. Quantification of positive cells from the Fig. 7E. G. Representative photographs of the expression patterns of miR-615-3p and SESN2 in tumor tissues from subcutaneous xenograft mouse model by IF and FISH. Scale bars = 20 μm. H. Representative photographs of the expression patterns of miR-615-3p and SESN2 in huamn OS tissues by IF and FISH. Scale bars = 20 μm. I. A negative correlation between the expression pattern of miR-615-3p and SESN2 (n = 40, r = -0.491, p < 0.01). Results are displayed as mean ± SD, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001