Fig. 7.

Validation of Hub Genes in Temperature treatment-associated DEGs. A: RT-qPCR was used to detect the level differences of AGT, BST2, COL3A1, GBP5, HSPA1A, HSPB1, IRF7, NLRC4, S100A4, and TLR7 mRNA in the 37 °C, 40 °C, and 44 °C groups (N = 4). Statistical analysis was completed using one-way ANOVA with Tukey’s multiple comparisons test, and P < 0.05 represents a significant difference. B: Correlation of RNA-seq and RT-qPCR results for hub genes (AGT, BST2, COL3A1, GBP5, HSPA1A, HSPB1, IRF7, NLRC4, S100A4, and TLR7) (N = 4). Statistical analysis was completed using Pearson correlation analysis, and P < 0.05 represents a significant difference