Fig. 4.

Effect of static mechanical compression on VAT explants. Gene expression levels of (A) PIEZO1, (B) SWELL1, (C) key inflammatory mediators and (H) extracellular matrix-related factors after 2 and 4 kPa of mechanical compression for 24 h. Secreted concentrations of the crucial inflammatory markers (D) IL-1α, (E) IL-1β, (F) IL-6 and (G) IL-8 in the culture media of human VAT explants after static compression for 24 h. Values are the mean ± SEM. Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests. ^*P < 0.05 and ^**P < 0.01. COL collagen; ELN elastin, IL interleukin, LOX lysyl oxidase, LRCC8A/SWELL1 leucine rich repeat containing 8 VRAC subunit A, MMP matrix metalloproteinase, NLRP nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin, SPP1 osteopontin, TLR4 toll-like receptor 4, TNF tumour necreosis factor-α