Fig. 7.

Static compression triggered an upregulation of PIEZO1 and SWELL1 expression levels in visceral adipose tissue (VAT) explants together with a strong increase in the expression and release of key inflammatory mediators (IL1A, IL1B, IL6, IL8, NLRP3, SPP1, TNF) and the downregulation of extracellular matrix factors (COL1A1, COL6A3, ELN). Obesity and obesity-associated T2D increased gene expression levels of PIEZO1 in VAT mainly due to adipocytes. SWELL1 and key markers of inflammation were also upregulated in VAT in obesity and T2D being significantly associated with PIEZO1 levels. The treatment of THP-1-derived macrophages with the secretome of adipocytes from patients with obesity upregulated PIEZO1 levels. COL collagen, ELN elastin, IL interleukin, NLRP nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin, SPP1 osteopontin, SWELL1 leucine rich repeat containing 8 VRAC subunit A, TNF tumour necrosis factor-α