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[Preprint]. 2024 Dec 9:2023.07.30.551157. [Version 2] doi: 10.1101/2023.07.30.551157

Fig. 6. Sphingomyelin drives cholesterol asymmetry.

Fig. 6.

(A) Schematic of proposed Chol redistribution induced by SMase. (B) Interleaflet Chol distributions in CG simulations of the simplified CG PM model from Fig. 2A before (purple) and after (orange) all SM has been converted to ceramide. (C) EGFP-GRAMH localization in RBL cells following SMase treatment; representative images on left, quantification on right. Each data point is a cell. (D) EGFP-GRAMH localization in RBL cells treated with 25 μM myriocin for 24 h; representative images on left, quantification on right. Small symbols represent individual cells, with symbol shapes denoting independent experiments. Filled larger symbols are means of independent experiments. Paired t-test on means of independent experiments; *p< 0.05.