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[Preprint]. 2024 Dec 12:2024.12.08.627273. [Version 1] doi: 10.1101/2024.12.08.627273

Fig. 1. Overview of PCLS cryopreservation.

Fig. 1.

(A) Previous works in the cryopreservation of PCLS report cooling rates that would lead to damaging ice formation. Our work uses high concentration cryoprotective agents and rapid cooling and rewarming to achieve ice-free vitrification. (B) Schematic diagram of the steps in the process for vitrification and rewarming using the cryomesh. CPA, cryopreservation agent.