Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

[Preprint]. 2024 Dec 12:2024.12.08.627273. [Version 1] doi: 10.1101/2024.12.08.627273

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.

PMC Copyright notice

Fig. 5. — (A) Acute evaluation of PCLS with AO/PI, H&E staining, and CYP1A1 zonated activity. (B) TUNEL staining after 24h culture to evaluate apoptosis (or necrosis) in both groups. Hematoxylin staining (blue) shows all nuclei and DAB stain (brown) shows TUNEL positive nuclei. (C) Quantification of TUNEL-positive cells (n=7). (D) Rates of resorufin production (N=3, n=4). The level of significance is presented by compact letter display (Kruskal-Walli’s test). AO, acridine orange; CYP1A1, Cytochrome P450 1A1; H&E, hematoxylin and eosin; PI, propidium iodide; VR, vitrification and rewarming; CV, central vein.

Fig. 5. — (A) Acute evaluation of PCLS with AO/PI, H&E staining, and CYP1A1 zonated activity. (B) TUNEL staining after 24h culture to evaluate apoptosis (or necrosis) in both groups. Hematoxylin staining (blue) shows all nuclei and DAB stain (brown) shows TUNEL positive nuclei. (C) Quantification of TUNEL-positive cells (n=7). (D) Rates of resorufin production (N=3, n=4). The level of significance is presented by compact letter display (Kruskal-Walli’s test). AO, acridine orange; CYP1A1, Cytochrome P450 1A1; H&E, hematoxylin and eosin; PI, propidium iodide; VR, vitrification and rewarming; CV, central vein.