Table 3. Cell activities in bovine mammary epithelial cells across both timepoints of 48 and 60 h after lysine or methionine subtraction to ¼ the normal concentration1.
Item | Treatment2 | SEM | P-value3 | |||
---|---|---|---|---|---|---|
CTL | LK | LM | LK | LM | ||
Protein synthesis rate | 272 | 213 | 186 | 11.5 | <0.01 | <0.01 |
Fat synthesis rate | 83.4 | 81.5 | 76.1 | 8.0 | 0.87 | 0.53 |
Cell proliferation rate | 13.2 | 10.5 | 8.4 | 0.8 | 0.03 | <0.01 |
PDI (arbitrary units) | 0.66 | 0.70 | 0.50 | 0.03 | 0.93 | 0.72 |
1Values are least squares means ± SE (n = 6) in dpm/ng DNA unless otherwise stated.
2CTL = control; LK = low lysine; LM = low methionine; PDI = endoplasmic reticulum tracker protein disulfide isomerase.
3Linear contrast of LK or LM vs. CTL.