Fig 1. Schematic representation of workflow, figured (by Figdraw https://www.figdraw.com/).

Antisense MO oligonucleotides were microinjected into fertilized one-cell stage embryos. At 2-dpf, zebrafish larvae were either photographed for phentying, or collected for single-cell preparation and sequencing using BD Rhapsody system. After filtering the raw data, further single-cell data analysis was performed such as cluster annotation, DEGs analysis, RNA velocity, cell-cell communication.