FIGURE 3.

Hamp ^−/− mice treated with CCl₄ had higher expression of F4/80, Ly6G, and CD45 than WT mice treated with CCl₄. (A) IHC of F4/80, (B) IHC of Ly6G, (C) IHC of CD45 in livers of WT and Hamp ^−/− mice with CCl₄-induced acute liver injury for 24 and 48 hours. (D) Positive F4/80 cells, (E) positive Ly6G cells, (F) positive CD45 cells in livers of WT and Hamp ^−/− mice with CCl₄-induced acute liver injury for 24 and 48 hours. Images of 10 random microscope fields of staining in each liver section were recorded for quantitative analysis using ImageJ software. *p < 0.05, **p < 0.01, compared with control. Abbreviations: CCl₄, carbon tetrachloride; Hamp ^−/−, hepcidin knockout; IHC, immunohistochemistry; WT, wild type.