FIGURE 6.

Hepcidin regulates the expression of Bcl-2 and Bax through PERK. (A) Volcano plot (p < 0.05 and FC >2 or FC <0.5 were differentially expressed. The red expressed upregulated genes, and the green expressed downregulated genes). (B) The expression of DEGs, (C, D) RNA-seq and RT-qPCR showed the expression of Eif2ak3/PERK in primary hepatocytes from WT and Hamp ^−/− mice treated with CCl₄. (E) The protein expression levels of P-PERK and PERK in WT and Hamp ^−/− mice with CCl₄-induced acute liver injury for 48 hours. (F) The mRNA expression of PERK in WT and Hamp ^−/− mice with CCl₄-induced liver fibrosis for 6 weeks. (G) The mRNA expression of Bax, (H) the mRNA expression of Bcl-2 after treated with TNFα, ActD, hepcidin peptide, PERK inhibitor in NCTC 1469. (I) The protein expression of BAX and BCL-2 after being treated with TNFα, ActD, hepcidin peptide, and PERK inhibitor in NCTC 1469. *p < 0.05, **p < 0.01, compared with control. Abbreviations: ActD, Dactinomycin; CCl₄, carbon tetrachloride; Hamp ^−/−, hepcidin knockout; WT, wild type.