Figure 2. The observation of PDGFR-α⁺ and NFATc1⁺ cells in maxilla M1 of Pdgfra^CreER × Nfatc1^DreER × LGRT mice (pulse) by whole-mount and high-speed imaging.

The whole-mount and high-speed imaging of (A) mouse molar with a tiling light-sheet microscope. (B) Schematic illustration of lineaging tracing in Pdgfra^CreER×Nfatc1^DreER× LGRT mice. The mice were administrated with tamoxifen at D1 and D3, and sacrificed at D5. (C) The contoured M1 of maxilla, including pulp and PDL with virtual dentin shell (white) in buccal view (scale bar = 300 μm). (D) Image stack was displayed in buccal view, coronal view, and radicular view of pulp and PDL, respectively (scale bar = 300 μm). (E) An optical slice was acquired on the X-Z (scale bar = 300 μm) and X-Y direction (scale bar = 400 μm) to display the pulp and PDL.

Figure 2—figure supplement 1. SUMIC procedure achieves whole-mount transparency of mice maxilla and enables imaging of dental, periodontal and alveolar bone.

(A) The tissue-clearing (TC) and whole-mount imaging procedure of mice maxilla. (B) The images before & after the TC procedure of mice maxilla. (C) The distribution of PDGFR-a⁺ and NFATc1⁺ cells from the section of XZ axis after 3D reconstruction of TC imaging. The image was from Pdgfra^CreER×Nfatc1^DreER× LGRT mice (pulse). Box 1: alveolar bone. Scale bar = 300 μm for top row, 100 μm for bottom row.

Figure 2—video 1. Panoptic multicolor imaging of PDGFR-α⁺ cells(green) & NFATc1⁺ cells(red) in the pulp and PDL area of maxilla M1 of Pdgfra^CreER×Nfatc1^DreER× LGRT mice (pulse), the whole-tissue imaging was achieved through TC procedure, related to Figure 2C.

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