Figure 3. Using traditional serial section for imaging of maxilla of Pdgfra^CreER×Nfatc1^DreER× LGRT mice (pulse).

(A) Operation process of frozen section. Using this procedure, a total of 121 slices were collected in this sample. (B) Representative images acquired by confocal microscopy (scale bar = 200 μm). Box 1: coronal pulp, Box 2: root pulp, Box 3: PDL, scale bar = 100 μm. (C) Maxilla M1 after 3D reconstruction by imaris, including pulp and PDL with virtual dentin shell (white) in buccal view (scale bar = 300 μm). (D) Image stack was displayed in buccal view, coronal view, and radicular view of pulp and PDL, respectively (scale bar = 300 μm).

Figure 3—figure supplement 1. The total 121 slices of maxilla M1 of Pdgfra^CreER×Nfatc1^DreER× LGRT mice sample (pulse).

The images were acquired by confocal microscope, ZsGreen⁺ cells in green, tdTomato⁺ cells in red, DAPI in blue.

Figure 3—figure supplement 2. Representative images of coronal pulp, root pulp, and PDL of maxilla M1 of Pdgfra^CreER×Nfatc1^DreER× LGRT mice sample (pulse).

The images were acquired by confocal microscope.

Figure 3—figure supplement 3. The tdTomato signal in pulp reconstructed by traditional serial section-based confocal imaging method (scale bar = 300 μm).

The sample was from Pdgfra^CreER×Nfatc1^DreER× LGRT mice (pulse).

Figure 3—figure supplement 4. The discontinuities in the z-axis due to stratification of slices.

The image was reconstructed by serial sections of maxilla M1 of Pdgfra^CreER×Nfatc1^DreER× LGRT mice sample (pulse).

Figure 3—figure supplement 5. 3D reconstruction of maxilla M1 of Pdgfra^CreER×Nfatc1^DreER× LGRT mice (pulse) by DICOM-3D; in PDL: ZsGreen⁺ cells in green, tdTomato⁺ cells in rose red; in pulp: ZsGreen⁺ cells in purple, tdTomato⁺ cells in blue.

The image stack was also displayed in buccal view, coronal view, and radicular view of pulp and PDL, respectively.

Figure 3—video 1. Panoptic multicolor imaging of PDGFR-α⁺ cells & NFATc1⁺ cells in the pulp and PDL area of maxilla M1 of Pdgfra^CreER×Nfatc1^DreER× LGRT mice (pulse), the whole-tissue imaging was reconstructed from serial sections, related to Figure 3C.

Download video file ^{(3MB, mp4)}

PDGFR-α⁺ cells in green, NFATc1⁺ cells in red and DAPI in blue.