Figure 8. IR1 tracing distinct cell populations simultaneously in the pulp and PDL.

(A) Schematic illustration of lineaging tracing in Pdgfra^CreER× IR1, Nfatc1^DreER× IR1 and Pdgfra^CreER×Nfatc1^DreER× IR1 mice. (B) Schematic diagram of the IR1 working principle. (C) 3D reconstruction of maxilla M1 using Imaris, including pulp and PDL with virtual dentin shell (white) (scale bar = 200 μm). The image stack was displayed in buccal view, coronal view, and radicular view of PDL (D) and pulp (E), respectively.

Figure 8—figure supplement 1. All consecutive slices (a total of 130 slices) for imaging of maxilla of Pdgfra^CreER × IR1 mice.

The images were acquired by confocal microscope, ZsGreen⁺ cells in green and DAPI in blue.

Figure 8—figure supplement 2. All consecutive slices (a total of 121 slices) for imaging of maxilla of Nfatc1^DreER× IR1 mice.

The images were acquired by confocal microscope, tdTomato⁺ cells in red and DAPI in blue.

Figure 8—figure supplement 3. All consecutive slices (a total of 127 slices) for imaging of maxilla of Pdgfra^CreER ×Nfatc1^DreER× IR1 mice sample.

The images were acquired by confocal microscope, ZsGreen⁺ cells in green, tdTomato⁺ cells in red, DAPI in blue.

Figure 8—figure supplement 4. Representative images of coronal pulp (A) and PDL (B) acquired by confocal microscope of mandible M1 of Pdgfra^CreER × IR1 (a1, b1), Nfatc1^DreER× IR1 (a2, b2) and Pdgfra^CreER×Nfatc1^DreER× IR1 (a3, b3) mice.

Scale bar = 100 μm.

Figure 8—figure supplement 5. 3D reconstruction of maxilla M1 by DICOM-3D of maxilla M1 of Nfatc1^DreER× IR1 and Pdgfra^CreER ×Nfatc1^DreER× IR1 mice.

(A) Schematic illustration of lineaging tracing in Nfatc1DreER× IR1 and PdgfraCreER×Nfatc1DreER× IR1 mice. (B) Schematic diagram of the IR1 working principle. (C-D) 3D reconstruction of maxilla M1 using DICOM-3D in Nfatc1DreER× IR1 (C) and PdgfraCreER×Nfatc1DreER× IR1 (D) mice, including pulp and PDL (scale bar = 200 μm). The image stack was displayed in buccal view, coronal view, andradicular view of PDL and pulp , respectively. In PDL, ZsGreen⁺ cells in green, tdTomato⁺ cells in rose red; in pulp, ZsGreen⁺ cells in purple, tdTomato⁺ cells in blue.

The image stack was displayed in buccal view, coronal view, and radicular view. Scale bar: 200 μm.

Figure 8—video 1. Panoptic multicolor imaging of ZsGreen⁺ cells in the pulp and PDL area of maxilla M1 of Pdgfra^CreER× IR1 mice, the whole-tissue imaging was reconstructed from serial sections, related to Figure 8.

Download video file ^{(2.3MB, mp4)}

ZsGreen⁺ cells in green and DAPI in blue.

Figure 8—video 2. Panoptic multicolor imaging of tdTomato⁺ cells in the pulp and PDL area of maxilla M1 of NFATc1^DreER× IR1 mice, the whole-tissue imaging was reconstructed from serial sections, related to Figure 8.

Download video file ^{(3.2MB, mp4)}

tdTomato⁺ cells in red and DAPI in blue.

Figure 8—video 3. Panoptic multicolor imaging of ZsGreen⁺ cells and tdTomato⁺ cells in the pulp and PDL area of maxilla M1 of Pdgfra^CreER×NFATc1^DreER× IR1 mice, the whole-tissue imaging was reconstructed from serial sections, related to Figure 8.

Download video file ^{(4.2MB, mp4)}

ZsGreen⁺ cells in green, tdTomato⁺ cells in red and DAPI in blue.