Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) | DNT2 Gal4 [CRISPR] | This study | Hidalgo Lab | See Materials and Methods, Molecular Biology and Figure 1. |
| Genetic reagent (D. melanogaster) | Toll-6 Gal4 [MIO2127] | Hidalgo Lab; Li et al., 2020 | Hidalgo Lab | |
| Genetic reagent (D. melanogaster) | kek-6 Gal4 [MI13953] | Hidalgo Lab; Ulian-Benitez et al., 2017 | Hidalgo Lab | |
| Genetic reagent (D. melanogaster) | Toll-2 Gal4 [pTV] | Hidalgo Lab; Li et al., 2020 | Hidalgo Lab | |
| Genetic reagent (D. melanogaster) | Toll-7 Gal4 [MI13963] | Hidalgo Lab; Li et al., 2020 | Hidalgo Lab | |
| Genetic reagent (D. melanogaster) | Toll-5 Gal4 [CRISPR] | Hidalgo Lab; Li et al., 2020 | Hidalgo Lab | |
| Genetic reagent (D. melanogaster) | Toll-8 Gal4 [MD806] | Bloomington Drosophila Stock Center (BDSC) | RRID:BDSC_36548 | |
| Genetic reagent (D. melanogaster) | MB320C-Gal4 (PPL1-ped) [1w[1118]; P{y[+t7.7] w[+mC]=R22B12-GAL4.DBD}attP2 PBac{y[+mDint2] w[+mC]=ple-p65.AD}VK00027118]; P{y[+t7.7] w[+mC]=R71D01-p65.AD}attP40; P{y[+t7.7] w[+mC]=R58F02-GAL4.DBD}attP2 |
BDSC | RRID:BDSC_68253 | |
| Genetic reagent (D. melanogaster) | MB301B-Gal4 (PAM-b2b'2) w[1118]; P{y[+t7.7] w[+mC]=R71D01-p65.AD}attP40; P{y[+t7.7] w[+mC]=R58F02-GAL4.DBD}attP2 |
BDSC | RRID:BDSC_68311 | |
| Genetic reagent (D. melanogaster) | TH-LexA(II) | Gift from Davis Ronald (via Serge Birman) | ||
| Genetic reagent (D. melanogaster) | TH-Gal4,R58E02-Gal4 | Gift from Serge Birman | ||
| Genetic reagent (D. melanogaster) | R58E02-Gal4 | BDSC | RRID:BDSC_41347 | |
| Genetic reagent (D. melanogaster) | R58E02-LexA | BDSC | RRID:BDSC_52740 | |
| Genetic reagent (D. melanogaster) | R14C08-LexA | BDSC | RRID:BDSC_52473 | |
| Genetic reagent (D. melanogaster) | Dop1R1-LexA | Gift from Deng et al., 2019 | ||
| Genetic reagent (D. melanogaster) | Dop2R-LexA | Gift from Deng et al., 2019 | ||
| Genetic reagent (D. melanogaster) | Gad-LexA | Gift from Yi Rao | ||
| Genetic reagent (D. melanogaster) | VT49239-LexA (DAL-LexA) | Gift from Ann-Shyn Chiang | ||
| Genetic reagent (D. melanogaster) | Tdc2-LexA | Gift from Carolina Rezaval | ||
| Genetic reagent (D. melanogaster) | Canton-S | Gift from Kei Ito | ||
| Genetic reagent (D. melanogaster) | Oregon | Hidalgo Lab | ||
| Genetic reagent (D. melanogaster) | MCFO: w[1118] P{y[+t7.7] w[+mC]=hs-FLPG5.PEST}attP3; P{y[+t7.7] w[+mC]=10xUAS(FRT.stop)myr::smGdP-OLLAS}attP2 PBac{y[+mDint2] w[+mC]=10xUAS(FRT.stop)myr::smGdP-HA}VK00005 P{10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG}su(Hw)attP1 | BDSC | RRID:BDSC_64086 | |
| Genetic reagent (D. melanogaster) | UAS-DNT2FL-GFP | Hidalgo Lab; Ulian-Benitez et al., 2017 | Hidalgo Lab | |
| Genetic reagent (D. melanogaster) | UAS-FlyBow1.1 (2609) | Gift from Iris Salecker (Hadjieconomou et al., 2011) | ||
| Genetic reagent (D. melanogaster) | hs Flp;;UAS-FlyBow2.0 | Gift from Iris Salecker (Ferrer et al., 1999; Hadjieconomou et al., 2011) | ||
| Genetic reagent (D. melanogaster) | 13xLexAop-nls-tdTomato | Gift from B. Pfeiffer | ||
| Genetic reagent (D. melanogaster) | UAS-DNT-2RNAi [VDRC49195] | VDRC | VDRC49195 | |
| Genetic reagent (D. melanogaster) | UAS-Toll6RNAi [P{GD35}v928] | VDRC | VDRC:928 FBst0471444 | |
| Genetic reagent (D. melanogaster) | UAS-Toll-6[CY] | Hidalgo Lab; McIlroy et al., 2013 | Hidalgo Lab | |
| Genetic reagent (D. melanogaster) | UAS-KeK6RNAi [KK-109681] | VDRC | VDRC:109681 FBgn0039862 | |
| Genetic reagent (D. melanogaster) | TransTango: UAS-myrGFP.QUAS-mtdTomato-3xHA (attp8); transTango (attp40) | BDSC | RRID:BDSC_77124 | |
| Genetic reagent (D. melanogaster) | 13LexAop-CD8::GFP, 10UAS-CD8::RFP | BDSC | RRID:BDSC_32229 | |
| Genetic reagent (D. melanogaster) | UAS-FB1.1 (406) | Gift from Iris Salecker (Hadjieconomou et al., 2011) | ||
| Genetic reagent (D. melanogaster) | UAS-DenMarkRFP, UAS-Dsyd1GFP | Gift from Carolina Rezaval | ||
| Genetic reagent (D. melanogaster) | MB247-Gal80 | BDSC | RRID:BDSC_64306 | |
| Genetic reagent (D. melanogaster) | DNT2[37]/TM6BlacZ | Hidalgo Lab; Ulian-Benitez et al., 2017 | ||
| Genetic reagent (D. melanogaster) | DNT2[18] | Hidalgo Lab; Ulian-Benitez et al., 2017 | ||
| Genetic reagent (D. melanogaster) | tubGal80[ts] on 2nd | BDSC | Hidalgo Lab | |
| Genetic reagent (D. melanogaster) | Toll-6[31]/TM6BlacZ | Hidalgo Lab (McIlroy et al., 2013) | ||
| Genetic reagent (D. melanogaster) | UAS-dop2R-RNAi [TRiP.HMC02988}attP40] | BDSC | RRID:BDSC_50621 | |
| Genetic reagent (D. melanogaster) | UAS-Toll6-RNAi [TRiP.HMS04251}attP2] | BDSC | RRID:BDSC_56048 | |
| Genetic reagent (D. melanogaster) | UAS-Brp-s-mCherry (II) | Gift from Stephan Sigrist | ||
| Genetic reagent (D. melanogaster) | UAS-Brp-s-mCherry (III) | Gift from Stephan Sigrist | ||
| Genetic reagent (D. melanogaster) | UAS-CD8::GFP (II) | Hidalgo Lab | ||
| Genetic reagent (D. melanogaster) | BacTrace 806: w; LexAop2-Syb::GFP-P10(VK37) LexAop-QF2::SNAP25::HIVNES::Syntaxin(VK18) / CyO; UAS-B3Recombinase(attP2) UAS<B3Stop <BoNT/A(VK5) | Cachero et al., 2020 | ||
| Genetic reagent (D. melanogaster) | UAS-UAS-Epac1-camps-50A | BDSC | RRID:BDSC_25408 | |
| Genetic reagent (D. melanogaster) | 20x UAS V5 Syn Cs Chrimson td tomato | Gift from B. Pfeiffer | ||
| Genetic reagent (D. melanogaster) | 13XLexAop2-IVS-GCaMP6s-p10 su(Hw)attP1 | BDSC | RRID:BDSC_44274 | |
| Genetic reagent (D. melanogaster) | 13XLexAop2-IVS-GCaMP6s-p10 su(Hw)attP5 | BDSC | RRID:BDSC_44590 | |
| Genetic reagent (D. melanogaster) | P{w[+mC]=UAS-syt.eGFP}2 | BDSC | RRID:BDSC_6925 | |
| Genetic reagent (D. melanogaster) | UAS-homer-GCaMP3 on 2nd | Gift from André Fialá | ||
| Antibody | Mouse monoclonal anti-brp (nc82) | Developmental Studies Hybridoma Bank (DSHB), IA | Cat# nc82; RRID:AB_2314866 |
1:10 |
| Antibody | Rabbit polyclonal anti-GFP | Thermo Fisher | Cat# A-11122; RRID:AB_221569 | 1:250 |
| Antibody | Mouse monoclonal anti-GFP | Thermo Fisher | Cat# A-11120; RRID:AB_221568 | 1:250 |
| Antibody | Chicken polyclonal anti-GFP | Aves | Cat# GFP1010; RRID:AB_2307313 | 1:500 |
| Antibody | Rabbit polyclonal anti-FLAG | Sigma | Cat# F7425 | 1:50 |
| Antibody | Mouse monoclonal anti-V5 | Invitrogen | Cat# # R960-25; RRID:AB_2556564 | 1:50 |
| Antibody | Chicken polyclonal anti-HA | Aves | Cat# ET-HA100; RRID:AB_2313511 | 1:50 |
| Antibody | Rabbit polyclonal anti-Vglut | Gift from Hermann | 1:500 | |
| Antibody | Mouse monoclonal anti-TH | Immunostar | Cat# 22941; RRID:AB_572268 | 1:250 |
| Antibody | Rabbit polyclonal anti-TH | Novus Biologicals | Cat# NB300-109 | 1:250 |
| Antibody | Rabbit polyclonal anti-DsRed | Clontek | Cat# 632496; RRID:AB_10013483 | 1:250 |
| Antibody | Mouse monoclonal anti-ChAT4B1 | DHSB |
Cat# ChAT4B1; RRID:AB_528122 |
1:250 |
| Antibody | Rabbit polyclonal anti-5-HT | Immunostar | Cat#20080; RRID:AB_572263 | 1:500 |
| Antibody | Rabbit polyclonal anti-DCP-1 | Cell Signalling | Cat# 8578S | 1:250 |
| Antibody | Alexa Flour 488 goat anti-mouse | Thermo Fisher | Cat# A-11001; RRID:AB_2534069 | 1:500 |
| Antibody | Alexa Flour 488 donkey anti-rabbit | Thermo Fisher | Cat# A-21206; RRID:AB_2435792 | 1:500 |
| Antibody | Alexa Flour 488 goat anti-rabbit (Fab')2 | Thermo Fisher | Cat# A-11070; RRID:AB_2534114 | 1:500 |
| Antibody | Alexa Flour 488 goat anti-chicken | Thermo Fisher | Cat# A-11039; RRID:AB_2534096 | 1:500 |
| Antibody | Alexa Four 546 goat anti-rabbit | Thermo Fisher | Cat# A-11035; RRID:AB_2534093 | 1:500 |
| Antibody | Alexa Four 546 goat anti-mouse | Thermo Fisher | Cat# A-11003; RRID:AB_25334071 | 1:500 |
| Antibody | Alex Four 647 goat anti-rabbit | Thermo Fisher | Cat# A-21245; RRID:AB_2535813 | 1:500 |
| Antibody | Alex Four 647 goat anti-mouse | Thermo Fisher | Cat# A-21236; RRID;AB_2535905 | :500 |
| Chemical compound, drug. | TRIzol | Ambion | Cat# AM9738 | |
| Commercial assay or kit | DNA-free DNA Removal Kit | Thermo Fisher | Cat# AM1906 | |
| Commercial assay or kit | GoScript Reverse Transcriptase | Promega | Cat# 237815 | |
| Chemical compound, drug | SensiFAST synGreen Mix | Bioline | Cat# B2092020 | |
| Chemical compound, drug | PFA | Sigma-Aldrich | Car# P6148 | |
| Chemical compound, drug | Triton X-100 | |||
| Chemical compound, drug | Normal goat serum | Vector Laboratories | Cat# S1000 | |
| Chemical compound, drug | 3-Octanol | Sigma-Aldrich | Cat# 218405 | |
| Chemical compound, drug | 4-Methylcyclohexaniol | Sigma-Aldrich | Cat# 153095 | |
| Chemical compound, drug | Mineral oil | Sigma-Aldrich | Cat# 330760 | |
| Commercial assay or kit | qPCR plate | GeneFlow | Cat# P3-0292 | |
| Software | Prism6 | GraphPad, CA | RRID:SCR_002798 | |
| Software | Fiji/ImageJ | Fiji | RRID:SCR_002285 | |
| Software | DeadEasy-Central Brain | Figure 3—source code 1. ImageJ plug-in originally published in Li et al., 2020 | DeadEasy_Central_Brain in UBIRA; https://edata.bham.ac.uk/ | |
| Software | DeadEasy-Dopaminergic neuron | Figure 3—source code 2. ImageJ plug-in | DeadEasy_Dopaminergic3DNew ni UBIRA; https://edata.bham.ac.uk/ |
|
| Software | Imaris | Bitplane | RRID:SCR_007370 | |
| Software | Adobe Illustrator | Adobe CS | RRID:SCR_010279 | |
| Software | Adobe Photoshop | Adobe CS | RRID:SCR_014199 | |
| Software; algorithm | FlyTracker | Eyrun Eyjolfsdottir, Steve Branson, Xavier P. Burgos-Artizzu, Eric D. Hoopfer, Jonathan Schor, David J Anderson, Pietro Perona. Eyjolfsdottir et al., 2014, Computer Vision – ECCV 772–787 |
https://kristinbranson.github.io/FlyTracker/ | |
| Software | MATLAB | MathWorks | RRID:SCR_001622 | |
| Other | Scope Fly Cell Atlas | Davie et al., 2018; Davie et al., 2018 | https://scope.aertslab.org/#/FlyCellAtlas/*/welcome |