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. 2024 Dec 20;15(12):923. doi: 10.1038/s41419-024-07276-3

Fig. 3. PI(4,5)P2 promotes IEC proliferation, inhibits death, and upregulates NNMT expression.

Fig. 3

A The viability of LPS-induced Caco-2 cells was detected by CCK8 upon PI(4,5)P2 treatment at the concentrations of 0, 12.5, 25, 50, 100, and 200 μM. B Dead cells were determined by flow cytometry. C The release of LDH level was measured by performing LDH assays. D The volcano plot of differentially expressed genes (DEGs) in blank and PI(4,5)P2-treated Caco-2 cells. E The bubble plot of the top 26 of KEGG pathway enrichment. F Seven DEGs with large fold changes, high expression, and upregulated in the PI(4,5)P2 group were selected for qRT-PCR verification. G The protein expression of NNMT in blank and PI(4,5)P2-treated Caco-2 cells was detected by WB. H The protein expression of NNMT in the colon mucosa of mice after PI(4,5)P2 treatment was detected by performing IHC. Scale bar, 100 μm. ns P > 0.05, ** P < 0.01, *** P < 0.001.