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. 2024 Dec 20;15(12):923. doi: 10.1038/s41419-024-07276-3

Fig. 4. NNMT regulates the function of PI(4,5)P2 in LPS-induced Caco-2 cells.

Fig. 4

NNMT overexpression efficiency was assessed by qRT-PCR (A) and WB (B). After NNMT overexpression, the viability (C), cell death (D), and LDH level (E) of Caco-2 cells were detected by CCK8, flow cytometry, and LDH assay, respectively. NNMT knockdown efficiency was assessed by qRT-PCR (F) and WB (G). After NNMT interference, cell death (H), the viability (I), and LDH level (J) of LPS-induced Caco-2 cells upon PI(4,5)P2 treatment were detected by CCK8, flow cytometry, and LDH assay, respectively. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.