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. 2024 Dec 20;15(12):923. doi: 10.1038/s41419-024-07276-3

Fig. 7. PI(4,5)P2 regulates the NNMT-RBP4 pathway to inhibit IEC pyroptosis.

Fig. 7

To identify PI(4,5)P2 suppresses pyroptosis in IECs depending on NNMT expression, after NNMT know down, the protein expression of caspase-1, GSDMD, and NLRP3 (A), IL-18 and IL-1β concentration (B), and LDH level (C) of LPS-induced Caco-2 cells upon PI(4,5)P2 treatment were detected by WB, ELISA, and LDH assay, respectively. RBP4 knockdown efficiency was assessed by qRT-PCR (D) and WB (E). Simultaneous transfection of si-NNMT and si-RBP4 into the PI(4,5)P2-treated Caco-2 cells, followed by detection of viability (F) and cell death (G) was detected by CCK8 and flow cytometry, respectively. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.