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. 2024 Dec 20;15(12):923. doi: 10.1038/s41419-024-07276-3

Fig. 8. Continuation of Fig. 7, to identify PI(4,5)P2 regulates the NNMT-RBP4 pathway to inhibit IEC pyroptosis.

Fig. 8

Simultaneous transfection of si-NNMT and si-RBP4 into the PI(4,5)P2-treated Caco-2 cells, and then the LDH level (A), protein expression levels of caspase-1, GSDMD, and NLRP3 (B), and IL-18 and IL-1β concentration (C) were detected by LDH assay, WB, and ELISA, respectively. D The expression of pyroptosis markers including caspase-1, GSDMD, and NLRP3 in the mucosa of CD mice after PI(4,5)P2 treatment was detected using IHC. Scale bar, 100 μm. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.