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. 2024 Dec 20;41(1):3. doi: 10.1007/s10565-024-09946-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — Extraction and identification of miR-21-Exo. (A) Transmission electron microscopy (n = 3) was utilized to investigate the structure of Exo and miR-21-Exo. (B) Exo and miR-21-Exo (n = 3) were analyzed with a Flow NanoAnalyzer to assess their particle sizes. (C) TSG101, CD81, and CD9 membrane protein levels in Exo and miR-21-Exo samples (n = 3) were measured. (D) The expression level of the miR-21 gene was assessed in BMSC and in Exo generated from BMSC transfected with miR-21 (n = 3). Standardization of the expression levels to U6 was done