Fig. 3.

miR-21-Exo enhanced the activity of CDDP-treated KGN cells. (A) CDDP-induced KGN cells (n = 3) were employed to observe the absorption of DiR-labeled Exo or miR-21-Exo using confocal microscopy. (B) Using the CCK8 test to calculate the proliferation rate (n = 3). (C) Using fluorescence-activated cell sorting to determine the rate of apoptosis (n = 3). (D) Measurement of estradiol concentration in cell supernatants using ELISA (n = 3). (E) qRT-PCR analysis (n = 3) of the mRNA levels of FSHR, CYP11A1, CYP19A1, HSD17B1, and STAR1, and normalized to GAPDH. (F) Quantification of LDH content in cell supernatants (n = 3). (G) Observation of cell morphology under electron microscopy. (H) Using Western Blotting and ImageJ software, the protein expressions of the pyroptosis-related genes NLRP3, CASP1, and GSDMD were analyzed (n = 3). (I) Using qRT-PCR (n = 3) and normalization to GAPDH, the mRNA levels of NLRP3, CASP1, GSDMD, IL1β, and IL18 were determined