Fig. 4.

miR-21-Exo regulated the MALT1 and NF-κB signaling pathways. (A) P The identification of pyroptosis-related MALT1 as a putative target gene of miR-21 via intersection (Data Sources: Tarbase, miRDB, DIANA tools, miRTarBase, miRWalk, rectome and GeneCards). (B) The expression of MALT1 mRNA is regulated by miR-21 (n = 3). (C) miRDB database predicts the specific binding site of miR-21 with MALT1 (n = 3). (D) Results from dual-luciferase reporter assays demonstrate the specific targeting of MALT1 by miR-21 (n = 3). The sequence's altered portion is denoted as MALT1-MUT. MALT1-WT denotes the wild-type MALT1 (n = 3). (E) Validation of the impact of miR-21 on the protein expression levels of the NF-κB signaling pathway through Western Blotting (n = 3)