Fig. 8.

Functional analysis of CEBPG in GBM cell viability, migration, and hypoxic response. A The knockdown efficiency of si-CEBPG in U87-MG and U118-MG tumor cell lines was measured by qRT-PCR experiment. B The cell viability of U87-MG and U118-MG after transfection with si-CEBPG was measured by CCK-8 assay at 0–72 h. C The migration ability of U87-MG and U118-MG cells at 0–72 h after transfection with si-CEBPG was assessed by the wound-healing assay. D The invasive ability of U87-MG and U118-MG cells after transfection with si-CEBPG was examined by transwell invasion assay. E 72 h after transfection of U87-MG and U118-MG cells with si-CEBPG, cells were harvested and stained with PI and Annexin V-FITC for apoptosis analysis. F The tumorigenicity of U87-MG and U118-MG cells after transfection with si-CEBPG was evaluated under normoxic and hypoxic conditions by colony formation assay