Figure 3.

Continued.

Figure 3.

MiR-487b-5p is low expression in AR patients and mediates expression of inflammatory cytokines. (A) The 5 candidate miRNAs have binding sites for hsa_circ_100791. (B and C) qRT-PCR analysis shows the expression level of 5 candidate miRNAs after being transfected with si-hsa_circ_100791 and NC or hsa_circ_100791 overexpression and control vector into HNEpCs. (D and E) qRT-PCR analysis shows that the miR-487b-5p was decreased in nasal mucosa of the AR patients. And statistical analysis indicated that negative correlation between hsa_circ_100791 and miR-487b-5p. (F) The qRT-PCR verified transfection efficiency of transfected the miR-487b-5p mimics or inhibitor into HNEpCs. (G–P) qRT-PCR analysis shows the mRNA expression level of IL-1β, IL-4, IL-5, IL-6, IL-8, IL-13, IL-17, IL-18, IL-33 and TNF-α was significantly reduced in miR-487b-5p mimics group, but obviously enhanced in miR-487b-5p inhibitor group. (Q and R) qRT-PCR analysis shows the mRNA expression level of IL-2, IFN-γ significantly enhanced in miR-487b-5p mimics group, but obviously reduced in miR-487b-5p inhibitor group. (S) Western blot analysis further revealed that the protein expression levels of IL-4, IL-5 and IL-13 was significantly reduced in miR-487b-5p mimics group, but obviously enhanced in miR-487b-5p inhibitor group. Data were presented as mean ± SEM. N= 3 for each group.

Abbreviation: NC, negative control.